Abstract
Because of the global spread of Zika virus, accurate and high-throughput diagnostic immunoassays are needed. We compared the sensitivity and specificity of 5 commercially available Zika virus serologic assays to the recommended protocol of Zika virus IgM-capture ELISA and plaque-reduction neutralization tests. Most commercial immunoassays showed low sensitivity, which can be increased.
Keywords:
Canada; ELISA; IgM; IgM-capture ELISA; MAC-ELISA; Zika virus; evaluation; immunoassays; sensitivity; specificity; vector-borne infections; viruses; zoonoses.
Publication types
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Comparative Study
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Evaluation Study
MeSH terms
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Antibodies, Viral / analysis
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Antibodies, Viral / immunology
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Enzyme-Linked Immunosorbent Assay / methods
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Humans
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Immunoassay / methods*
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Immunoglobulin G / analysis
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Immunoglobulin G / immunology
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Immunoglobulin M / analysis
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Immunoglobulin M / immunology
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Neutralization Tests / methods
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Sensitivity and Specificity
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Zika Virus / immunology
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Zika Virus / isolation & purification*
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Zika Virus Infection / diagnosis
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Zika Virus Infection / virology*
Substances
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Antibodies, Viral
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Immunoglobulin G
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Immunoglobulin M