During 2014-2016, we conducted mosquito-based Zika virus surveillance in Rio de Janeiro, Brazil. Results suggest that Zika virus was probably introduced into the area during May-November 2013 via multiple in-country sources. Furthermore, our results strengthen the hypothesis that Zika virus in the Americas originated in Brazil during October 2012-May 2013.
Keywords: Aedes aegypti; Brazil; Germany; Zika virus; introduction; lineage; mosquito; phylogeny; surveillance; vector-borne infections; viruses.