Efficacy of novel antibody-based drugs against rhinovirus infection: In vitro and in vivo results

Antiviral Res. 2017 Jun:142:185-192. doi: 10.1016/j.antiviral.2017.03.017. Epub 2017 Mar 27.

Abstract

Rhinoviruses (RVs) cause the common cold and are associated with exacerbations of chronic inflammatory respiratory diseases, especially asthma and chronic obstructive pulmonary disease (COPD). We have assessed the antiviral drugs Anaferon for Children (AC) and Ergoferon (containing AC as one of the active pharmaceutical ingredients) in in vitro and in vivo experimental models, in order to evaluate their anti-rhinoviral and immunomodulatory potential. HeLa cells were pretreated with AC, and levels of the interferon-stimulated gene (ISG), 2'-5'-oligoadenylate synthetase 1 (OAS1-A) and viral replication were analyzed. In a mouse model of RV-induced exacerbation of allergic airway inflammation we administered Ergoferon and analyzed its effect on type I (IFN-β), type II (IFN-γ) and type III (IFN-λ) IFNs induction, cell counts in bronchoalveolar lavage (BAL), cytokine (interleukin (IL)-4; IL-6) and chemokine (CXCL10/IP-10; CXCL1/KC) levels. It was shown that AC increased OAS1-А production and significantly decreased viral replication in vitro. Increased IFNs expression together with reduced neutrophils/lymphocytes recruitment and correlated IL-4/IL-6 declination was demonstrated for Ergoferon in vivo. However, there was no effect on examined chemokines. We conclude that AC and Ergoferon possess effects against RV infection and may have potential as novel therapies against RV-induced exacerbations of asthma.

Keywords: Antibodies; Cytokine; Human rhinovirus infection; Interferon stimulated genes.

Publication types

  • Research Support, Non-U.S. Gov't
  • Retracted Publication

MeSH terms

  • 2',5'-Oligoadenylate Synthetase / analysis
  • Animals
  • Antibodies / pharmacology*
  • Antiviral Agents / immunology*
  • Antiviral Agents / pharmacology*
  • Asthma / immunology
  • Asthma / virology
  • Cell Line
  • Chemokines / metabolism
  • Child
  • Cytokines / drug effects
  • Cytokines / metabolism
  • Disease Models, Animal
  • Gene Expression / drug effects
  • HeLa Cells
  • Humans
  • Inflammation
  • Interferons / drug effects
  • Mice
  • Mice, Inbred BALB C
  • Picornaviridae Infections / drug therapy*
  • Respiratory System / immunology
  • Respiratory System / virology
  • Rhinovirus / drug effects*
  • Rhinovirus / pathogenicity

Substances

  • Antibodies
  • Antiviral Agents
  • Chemokines
  • Cytokines
  • anaferon
  • ergoferon
  • Interferons
  • 2',5'-Oligoadenylate Synthetase