Abstract
We evaluated the safety and efficacy of a live attenuated influenza vaccine (LAIV) product in ferrets. The BCHT LAIV product was significantly less virulent than wild-type H1N1 virus, when evaluated by comparing virus shedding and histopathologic lesions. The data indicated strong evidence for an attenuated phenotype of LAIV. Furthermore, the vaccine induced robust humoral immune responses in seronegative ferrets, and protected ferrets against development of fever, weight loss and turbinate inflammatory lesions after challenging with H3N2 wide-type influenza virus. Thus, the BCHT LAIV product was safe in healthy seronegative ferrets and protected ferrets against infection of H3N2 influenza virus.
Keywords:
Ferret; Influenza; Trivalent live attenuated influenza vaccine.
Copyright © 2016 Elsevier Ltd. All rights reserved.
MeSH terms
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Animals
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Antibodies, Viral / blood
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China
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Ferrets
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Humans
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Immunity, Humoral
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Influenza A Virus, H1N1 Subtype / immunology
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Influenza A Virus, H1N1 Subtype / isolation & purification
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Influenza A Virus, H1N1 Subtype / pathogenicity
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Influenza A Virus, H3N2 Subtype / immunology*
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Influenza A Virus, H3N2 Subtype / isolation & purification
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Influenza A Virus, H3N2 Subtype / pathogenicity
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Influenza A Virus, H3N2 Subtype / physiology
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Influenza B virus / immunology
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Influenza B virus / isolation & purification
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Influenza Vaccines / administration & dosage
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Influenza Vaccines / adverse effects*
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Influenza Vaccines / immunology*
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Influenza, Human / prevention & control
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Male
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Orthomyxoviridae Infections / immunology
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Orthomyxoviridae Infections / prevention & control*
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Vaccines, Attenuated / administration & dosage
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Vaccines, Attenuated / adverse effects
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Vaccines, Attenuated / immunology
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Virus Shedding
Substances
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Antibodies, Viral
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Influenza Vaccines
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Vaccines, Attenuated