Rapid molecular diagnostic test for Zika virus with low demands on sample preparation and instrumentation

Kevin E Eboigbodin; Mirko Brummer; Tuomas Ojalehto; Mark Hoser

doi:10.1016/j.diagmicrobio.2016.08.027

Rapid molecular diagnostic test for Zika virus with low demands on sample preparation and instrumentation

Diagn Microbiol Infect Dis. 2016 Dec;86(4):369-371. doi: 10.1016/j.diagmicrobio.2016.08.027. Epub 2016 Sep 2.

Authors

Kevin E Eboigbodin¹, Mirko Brummer², Tuomas Ojalehto², Mark Hoser³

Affiliations

¹ Research and Development, Orion Diagnostica Oy, P. O. BOX 83, FI-02101 Espoo, Finland. Electronic address: kevin.eboigbodin@oriondiagnostica.fi.
² Research and Development, Orion Diagnostica Oy, P. O. BOX 83, FI-02101 Espoo, Finland.
³ Molecular Biology, GeneForm Technologies, Broadstairs, United Kingdom.

PMID: 27645608
DOI: 10.1016/j.diagmicrobio.2016.08.027

Abstract

Zika virus has only recently gained attention due to recent large outbreaks worldwide. An easy to use nucleic acid amplification test could play an important role in the early detection of the infection and patient management. Here, we report a rapid and robust isothermal nucleic acid amplification assay for the detection of Zika virus. The method is cost-effective and compatible with portable instrumentation, enabling near patient testing and field use.

Keywords: Amplification; Diagnostics; Isothermal; Outbreak; Portable; RNA; Virus; Zika.

Publication types

Evaluation Study

MeSH terms

Cost-Benefit Analysis
Humans
Molecular Diagnostic Techniques / methods*
Nucleic Acid Amplification Techniques / methods*
Time Factors
Zika Virus / genetics
Zika Virus / isolation & purification*
Zika Virus Infection / diagnosis*