Abstract
The terminal noncoding region (NCR) sequences of the eight gene segments of the influenza A/Brevig Mission/1/1918 (H1N1) virus were determined by rapid amplification of cDNA ends (RACE). Chimeric viruses encoding the open reading frames of the 1918 virus but flanked by either the wild-type 1918 NCR sequences or the NCR sequences of two other H1N1 virus strains, A/WSN/1933 and A/New York/312/2001, were produced. No growth differences between the NCR variant 1918 influenza viruses were noted.
Publication types
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Research Support, N.I.H., Intramural
MeSH terms
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Base Sequence
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Cell Line
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Humans
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Influenza A Virus, H1N1 Subtype / chemistry
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Influenza A Virus, H1N1 Subtype / genetics*
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Influenza A Virus, H1N1 Subtype / growth & development
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Influenza A Virus, H1N1 Subtype / physiology
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Influenza, Human / virology*
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Molecular Sequence Data
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Open Reading Frames
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RNA, Viral / chemistry
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RNA, Viral / genetics*
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RNA, Viral / metabolism
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Untranslated Regions*
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Virus Replication
Substances
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RNA, Viral
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Untranslated Regions