Abstract
Influenza virus rgVac1sub-P0 (H5N1) (rgVac1-P0), in which a pair of dibasic amino acid residues was introduced at the cleavage site of the HA of a reassortant of H5N2 and H7N1 viruses of duck origin, was low pathogenic in chickens. Vac1sub-P3 (H5N1) (Vac1-P3) was selected as a highly pathogenic avian influenza virus by 3 consecutive passages in chickens from low pathogenic strain rgVac1-P0. Comparison of amino acid sequences of the virus proteins and experimental infection of chickens with a series of recombinant viruses demonstrated that in addition to the HA, each of the PA, NP, M1, and M2 of Vac1-P3 are responsible for the acquisition of pathogenicity in chickens. These 4 proteins of Vac1-P3 synergistically contributed to efficient virus replication in chickens.
Copyright © 2013 Elsevier B.V. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Chickens
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Ducks
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Influenza A Virus, H5N1 Subtype / genetics
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Influenza A Virus, H5N1 Subtype / isolation & purification
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Influenza A Virus, H5N1 Subtype / pathogenicity*
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Influenza A Virus, H5N2 Subtype / genetics
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Influenza A Virus, H5N2 Subtype / isolation & purification
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Influenza A Virus, H5N2 Subtype / pathogenicity*
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Influenza A Virus, H7N1 Subtype / genetics
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Influenza A Virus, H7N1 Subtype / isolation & purification
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Influenza A Virus, H7N1 Subtype / pathogenicity*
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Influenza in Birds / pathology
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Influenza in Birds / virology*
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Reassortant Viruses / genetics
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Reassortant Viruses / isolation & purification
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Reassortant Viruses / pathogenicity*
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Viral Proteins / genetics
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Viral Proteins / metabolism*
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Virulence
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Virulence Factors / genetics
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Virulence Factors / metabolism*
Substances
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Viral Proteins
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Virulence Factors