Comparison of neutralizing and hemagglutination-inhibiting antibody responses for evaluating the seasonal influenza vaccine

J Virol Methods. 2012 Jun;182(1-2):43-9. doi: 10.1016/j.jviromet.2012.03.004. Epub 2012 Mar 13.

Abstract

Evaluation of the efficacy of influenza vaccines is essential for vaccine development. This study evaluated the neutralizing and hemagglutination-inhibition antibody response in subjects receiving the 2006-07 and 2007-08 seasonal influenza vaccines. ELISA-based microneutralization demonstrated a greater mean-fold increase and seroconversion rate than the hemagglutination-inhibition assay. The increase in the antibody titers against influenza H1 were higher than those against influenza H3 and influenza B, indicating that the H1 vaccine strain in the 2006-07 and 2007-08 seasons was more immunogenic. These data suggest that the neutralizing antibody response is a better measurement of influenza vaccine efficacy.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Antibodies, Neutralizing / blood
  • Antibodies, Viral / blood*
  • Child
  • Child, Preschool
  • Enzyme-Linked Immunosorbent Assay / methods
  • Female
  • Hemagglutination Tests / methods*
  • Humans
  • Infant
  • Infant, Newborn
  • Influenza Vaccines / administration & dosage
  • Influenza Vaccines / immunology*
  • Male
  • Middle Aged
  • Neutralization Tests / methods*
  • Sensitivity and Specificity
  • Young Adult

Substances

  • Antibodies, Neutralizing
  • Antibodies, Viral
  • Influenza Vaccines